240 mM glycine. Cells had been washed, resuspended in sonication buffer (10 mM Tris-HCl
240 mM glycine. Cells have been washed, resuspended in sonication buffer (10 mM Tris-HCl (pH eight.0), 1 mM EDTA, 0.five mM EGTA, 0.5 mM PMSF, 1 SDS) and sonicated on…